COMPARATIVE EFFICIENCY OF TWO FREEZING EXTENDERS FOR BOVINE SEMEN BASED ON SPERM MOTILITY AND MEMBRANE INTEGRITY.

Authors

  • A. M. CRESPILHO Faculdade
  • F. O. PAPA Faculdade
  • K. ALBERTI Faculdade
  • E. R. SIQUEIRA FILHO Faculdade
  • A. MARTINS JR Faculdade
  • J. L. C. NOVAES Faculdade
  • J. A. DELL'AQUA Faculdade

DOI:

https://doi.org/10.15361/2175-0106.2006v22n3p229-235

Abstract

The quality of the seminal samples has an important role in the determination of the indexes of fertility in artificial insemination programs. In that sense, the aim of the study was to compare the efficacy of 2 extenders for cryopreservation of bull semen in relation to the motility patterns post-thaw evaluated by computer-assisted sperm analysis (CASA), the integrity of plasmatic membrane (IMP) and viability to the fast term-resistance test (TTR)roportionate for each one of the freezing methodology. For the study 10 bulls of the Nelore breed were used, obtaining two ejaculated of each animal. Ejaculates were then splited in two batches, submitted to cryopreservation with the extenders Botu-Bov® (Challenge group) and Tris-egg yolk-fructose (Control group). The data were analyzed by the software Grafpad Instat 5.0, comparing the averages obtained through the

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Published

20/11/2008

Issue

Vol. 22 No. 3 (2006)

Section

Animal Reproduction/Reprodução Animal

License

Authors must be aware that in case the manuscript is accepted for publication, the copyright is transferred to FUNEP. Total or partial reproduction is allowed provided that the reference is indicated explicitly.